Skagias L, Politi E, Karameris A, Sambaziotis D, Archondakis A, Vasou O, Ntinis A, Michalopoulou F, Moreas I, Koutselini H, Patsouris E
Department of Cytopathology, Aretaieion University Hospital, Athens, Greece.
J BUON. 2009 Jul-Sep;14(3):457-62.
This study was conducted to evaluate the quantitative assessment of HER2/neu immunohistochemical expression in urothelial bladder cancer in order to determine its prognostic significance.
Archival tumor tissue from 80 patients with primary urothelial carcinoma were analysed for HER2/neu immunohistochemical expression. A highly reproducible standardized procedure on a Bond-X automated slide stainer was used.
HER2 protein was overexpressed in 41 of 80 patients (51.25%), demonstrating an increase in the expression rate corresponding to progressively advanced tumor stage (p=0.032) and tumor grade (p=0.0001). Kaplan-Meier analyses showed that positive membranous expression of HER2/neu was not associated with an increased probability of tumor recurrence (p=0.362). In contrast, HER2 scores correlated strongly with specific survival probability (p=0.002) and overall survival (p=0.025). Multivariate analysis revealed that only stage was an independent predictor of specific survival (p=0.016). HER2 expression was an independent predictor of specific survival with borderline statistical significance (p=0.08).
HER2 overexpression represents a prognostic factor for adverse disease outcome.
本研究旨在评估尿路上皮膀胱癌中HER2/neu免疫组化表达的定量评估，以确定其预后意义。
对80例原发性尿路上皮癌患者的存档肿瘤组织进行HER2/neu免疫组化表达分析。在Bond-X自动玻片染色机上采用高度可重复的标准化程序。
80例患者中有41例（51.25%）HER2蛋白过度表达，显示表达率随肿瘤分期进展（p=0.032）和肿瘤分级升高（p=0.0001）而增加。Kaplan-Meier分析表明，HER2/neu的阳性膜表达与肿瘤复发概率增加无关（p=0.362）。相反，HER2评分与特定生存概率（p=0.002）和总生存（p=0.025）密切相关。多因素分析显示，只有分期是特定生存的独立预测因素（p=0.016）。HER2表达是特定生存的独立预测因素，具有临界统计学意义（p=0.08）。
HER2过表达是疾病不良预后的一个因素。
