Vrabec Branica Bozica, Mitrecić Dinko, Grgić Sandra, Smojver Jezek Silvana, Chalfe Nabil, Gajović Srećko
Department of Cytology, University Hospital for Lung Diseases Jordanovac, Zagreb, Croatia.
Genet Test Mol Biomarkers. 2009 Dec;13(6):799-802. doi: 10.1089/gtmb.2009.0066.
There are only a few systematic reports about DNA extraction from routine diagnostic cytological specimens. An inevitable drawback of such techniques is increased spending of time and funds required for obligatory DNA purification. To implement a simple protocol for human DNA isolation from cytological specimens related to lung cancer, bronchial aspirates together with samples collected by swabbing of the inner cheek and eyelid were used. By combining alkaline and temperature lyses it was possible to isolate DNA solution ready for PCR in less than an hour. Testing the method used for amplification of sex chromatin gene fragments showed that it is highly efficient. The presented protocol preserves high-quality DNA that is suitable for PCR-based assays.
关于从常规诊断细胞学标本中提取DNA的系统报告很少。此类技术不可避免的一个缺点是，进行必要的DNA纯化需要增加时间和资金投入。为了实施一种从与肺癌相关的细胞学标本中分离人DNA的简单方案，使用了支气管吸出物以及通过擦拭脸颊内侧和眼睑收集的样本。通过结合碱性裂解和温度裂解，能够在不到一小时的时间内分离出可供PCR使用的DNA溶液。对用于扩增性染色质基因片段的方法进行测试表明，该方法效率很高。所提出的方案可保留适用于基于PCR分析的高质量DNA。
