Borenfreund E, Higgins P J, Bendich A
Cancer Lett. 1977 Sep;3(3-4):145-50. doi: 10.1016/s0304-3835(77)95126-6.
Epithelial liver cells isolated from rats after oral diethylnitrosamine administration were established in culture. When propagated in vitro for 2--3 months, over half the cells contained acentric nuclei and large juxtanuclear inclusions resembling the hyaline deposits (Mallory bodies) in cirrhotic livers of alcoholics. The morphological changes and disarrayed filaments in these bodies were retained on serial passages for many months. Cells inoculated into nude mice or newborn rats produced carcinomas from which cells with these abnormalities were reestablished in continuous culture.
从经口给予二乙基亚硝胺的大鼠中分离出的肝上皮细胞被进行培养。当在体外传代培养2至3个月时，超过半数的细胞含有无着丝粒核以及类似于酒精性肝硬化肝脏中透明样沉积物（马洛里小体）的大的核旁包涵体。这些包涵体中的形态学变化和紊乱的细丝在连续传代许多个月后仍得以保留。接种到裸鼠或新生大鼠体内的细胞产生了癌，从这些癌中又重新建立起具有这些异常的细胞并进行连续培养。
