Sharma Hari Shanker, Zimmermann-Meinzingen Sibilla, Sharma Aruna, Johanson Conrad E
Laboratory of Carebrovascular Research, Department of Surgical Science, Anesthesiology and Intensive Care Medicine, University Hospital, Uppsala, Sweden.
Acta Neurochir Suppl. 2010;106:321-5. doi: 10.1007/978-3-211-98811-4_60.
The possibility that Cerebrolysin, a mixture of several neurotrophic factors, has some neuroprotective effects on whole body hyperthermia (WBH) induced breakdown of the blood-brain barrier (BBB), blood-CSF barrier (BCSFB), brain edema formation and neuropathology were examined in a rat model. Rats subjected to a 4 h heat stress at 38 degrees C in a biological oxygen demand (BOD) incubator exhibited profound increases in BBB and BCSFB permeability to Evans blue and radioiodine tracers compared to controls. Hippocampus, caudate nucleus, thalamus and hypothalamus exhibited pronounced increase in water content and brain pathology following 4 h heat stress. Pretreatment with Cerebrolysin (1, 2 or 5 mL/kg i.v.) 24 h before WBH significantly attenuated breakdown of the BBB or BCSFB and brain edema formation. This effect was dose dependent. Interestingly, the cell and tissue injury following WBH in cerebrolysin-treated groups were also considerably reduced. These novel observations suggest that cerebrolysin can attenuate WBH induced BBB and BCSFB damage resulting in neuroprotection.
在大鼠模型中，研究了脑蛋白水解物（几种神经营养因子的混合物）对全身热疗（WBH）诱导的血脑屏障（BBB）破坏、血脑脊液屏障（BCSFB）破坏、脑水肿形成及神经病理学是否具有神经保护作用。与对照组相比，在生物需氧量（BOD）培养箱中于38℃进行4小时热应激的大鼠，其BBB和BCSFB对伊文思蓝和放射性碘示踪剂的通透性显著增加。热应激4小时后，海马、尾状核、丘脑和下丘脑的含水量显著增加，且出现脑病理学改变。在WBH前24小时静脉注射脑蛋白水解物（1、2或5 mL/kg）预处理，可显著减轻BBB或BCSFB的破坏以及脑水肿的形成。这种作用呈剂量依赖性。有趣的是，脑蛋白水解物治疗组在WBH后的细胞和组织损伤也明显减轻。这些新发现表明，脑蛋白水解物可减轻WBH诱导的BBB和BCSFB损伤，从而产生神经保护作用。
