Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Histopathology. 2009 Oct;55(4):414-22. doi: 10.1111/j.1365-2559.2009.03403.x.
Lynch syndrome-associated tumours are characterized by the presence of an increased number of tumour-infiltrating lymphocytes. This enhanced lymphocytic response may be elicited by genetically altered proteins that may arise as a result of a defective DNA mismatch repair system. The aim was to investigate this hypothesis by correlating loss of mismatch repair proteins and infiltration of lymphocytes in Lynch syndrome-associated adenomas and hyperplastic polyps.
Mismatch repair protein expression and the number of tumour-infiltrating lymphocytes were assessed in Lynch syndrome (41 adenomas and nine hyperplastic polyps) and in familial colorectal cancer (nine adenomas and one hyperplastic polyp). Nineteen sporadic adenomas were included as a control group. Twenty of 32 (63%) adenomas with loss of mismatch repair protein expression showed an increase in tumour-infiltrating lymphocytes. Eight adenomas (8/32; 25%) displayed many tumour-infiltrating lymphocytes, whereas most adenomas (12/32; 38%) showed a minor increase. In adenomas with mismatch repair protein expression, both sporadic and Lynch syndrome associated, not one showed an increased number of tumour-infiltrating lymphocytes. Hyperplastic polyps in Lynch syndrome patients showed neither loss of mismatch repair expression nor an increase in tumour-infiltrating lymphocytes.
There is a correlation between the loss of mismatch repair proteins and the infiltration of lymphocytes in Lynch syndrome-associated adenomas.
林奇综合征相关肿瘤的特征是存在大量肿瘤浸润淋巴细胞。这种增强的淋巴细胞反应可能是由基因突变蛋白引起的，这些基因突变蛋白可能是由于 DNA 错配修复系统缺陷而产生的。本研究旨在通过相关性分析来验证这一假设，即林奇综合征相关腺瘤和增生性息肉中错配修复蛋白缺失与淋巴细胞浸润的关系。
评估了林奇综合征（41 个腺瘤和 9 个增生性息肉）和家族性结直肠癌（9 个腺瘤和 1 个增生性息肉）中错配修复蛋白的表达和肿瘤浸润淋巴细胞的数量。作为对照组，纳入了 19 个散发性腺瘤。在 32 个缺失错配修复蛋白表达的腺瘤中，有 20 个（63%）显示肿瘤浸润淋巴细胞增多。8 个（8/32；25%）腺瘤中有大量肿瘤浸润淋巴细胞，而大多数（12/32；38%）腺瘤仅有少量增加。在表达错配修复蛋白的腺瘤中，无论是散发性的还是林奇综合征相关的，都没有一个表现出肿瘤浸润淋巴细胞数量增加。林奇综合征患者的增生性息肉既没有缺失错配修复蛋白表达，也没有增加肿瘤浸润淋巴细胞。
林奇综合征相关腺瘤中错配修复蛋白缺失与淋巴细胞浸润之间存在相关性。
