Xu R K
Institute of Basic Medical Sciences, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1990 Jun;12(3):178-81.
To determine the role of central endogenous dynorphin (Dyn) in resting and stress-induced release of ACTH and prolactin (PRL), Dyn (1-17) antiserum (AS) or normal rabbit serum (NRS) were microinjected into the 3rd ventricle of freely-moving male rats. Plasma ACTH and PRL levels were measured by radioimmunoassay (RIA) using RIA kits supplied by NIADDK. Our results clearly indicate that Dyn (1-17) AS and NRS had no effect on resting secretion of ACTH and PRL, and restraint stress-induced release of ACTH and PRL were simultaneously lowered significantly in the Dyn (1-17) AS-treated group as compared with the NRS-treated group. The results suggest that central endogenous dynorphin plays an important role in stimulating the concomitant release of ACTH and PRL under certain stresses.
为确定中枢内源性强啡肽（Dyn）在静息及应激诱导的促肾上腺皮质激素（ACTH）和催乳素（PRL）释放中的作用，将Dyn（1 - 17）抗血清（AS）或正常兔血清（NRS）微量注射到自由活动雄性大鼠的第三脑室。使用美国国立糖尿病、消化和肾脏疾病研究所（NIADDK）提供的放射免疫分析（RIA）试剂盒，通过放射免疫分析法测定血浆ACTH和PRL水平。我们的结果清楚地表明，Dyn（1 - 17）AS和NRS对ACTH和PRL的静息分泌没有影响，与NRS处理组相比，Dyn（1 - 17）AS处理组中束缚应激诱导的ACTH和PRL释放同时显著降低。结果表明，中枢内源性强啡肽在特定应激下刺激ACTH和PRL的伴随释放中起重要作用。
