Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Bioresour Technol. 2010 Feb;101(3):1102-5. doi: 10.1016/j.biortech.2009.08.058. Epub 2009 Oct 9.
A new dibenzothiophene (DBT) desulfurizing bacterium was isolated from oil-contaminated soils in Iran. HPLC analysis and PCR-based detection of the presence of the DBT desulfurization genes (dszA, dszB and dszC) indicate that this strain converts DBT to 2-hydroxybiphenyl (2-HBP) via the 4S pathway. The strain, identified as Rhodococcus erythropolis SHT87, can utilize DBT, dibenzothiophene sulfone, thiophene, 2-methylthiophene and dimethylsulfoxide as a sole sulfur source for growth at 30 degrees C. The maximum specific desulfurization activity of strain SHT87 resting cells in aqueous and biphasic organic-aqueous systems at 30 degrees C was determined to be 0.36 and 0.47 micromol 2-HBP min(-1) (gdrycell)(-1), respectively. Three mM DBT was completely metabolized by SHT87 resting cells in the aqueous and biphasic systems within 10h. The rate and the extent of the desulfurization reaction by strain SHT87 suggest that this strain can be used for the biodesulfurization of diesel oils.
从伊朗受污染土壤中分离出一种新的二苯并噻吩（DBT）脱硫菌。HPLC 分析和基于 PCR 的 DBT 脱硫基因（dszA、dszB 和 dszC）检测表明，该菌株通过 4S 途径将 DBT 转化为 2-羟基联苯（2-HBP）。该菌株被鉴定为红球菌 erythropolis SHT87，能够利用 DBT、二苯并噻吩砜、噻吩、2-甲基噻吩和二甲基亚砜作为唯一的硫源，在 30°C 下生长。在 30°C 下，SHT87 静止细胞在水相和两相有机-水相体系中的最大比脱硫活性分别为 0.36 和 0.47µmol 2-HBP min-1（gdrycell）-1。在水相和两相体系中，3mM 的 DBT 在 10 小时内被 SHT87 静止细胞完全代谢。SHT87 菌株的脱硫反应速率和程度表明，该菌株可用于柴油的生物脱硫。
