检测马匹短期口服后尿液和血液中的克伦特罗。
Detection of urine and blood clenbuterol following short-term oral administration in the horse.
机构信息
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.
出版信息
Immunopharmacol Immunotoxicol. 2010 Mar;32(1):171-6. doi: 10.3109/08923970903179688.
BACKGROUND AND AIM
The pharmacokinetics of clenbuterol in equine urine and blood was investigated.
MATERIAL AND METHODS
Urine and blood samples were collected following 3-day multiple oral administrations. The samples were examined using enzyme-linked immunosorbent assay and further confirmed by solid phase extraction and capillary electrophoresis.
RESULTS
Urinary clenbuterol was detectable until day 14 after the last dose. The urinary excretion of clenbuterol was characterized by a biphasic pattern. The half-lives of the bi-exponential elimination (t(1/2alpha) and t(1/2beta)) for urinary clenbuterol were about 12.1 and 48 hours. After a single oral administration (4 microg/kg) of clenbuterol, the half-life of serum clenbuterol was approximately 11.4 hours.
背景与目的
研究了克仑特罗在马尿液和血液中的药代动力学。
材料与方法
在连续 3 天多次口服给药后采集尿液和血液样本。使用酶联免疫吸附测定法（ELISA）进行检测，并用固相萃取和毛细管电泳进一步确认。
结果
末次给药后 14 天内仍可检测到尿液中的克仑特罗。尿液中克仑特罗的排泄呈双相模式。尿液中克仑特罗的双指数消除半衰期（t(1/2alpha)和 t(1/2beta)）约为 12.1 和 48 小时。单次口服（4μg/kg）克仑特罗后，血清中克仑特罗的半衰期约为 11.4 小时。
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