Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Artif Organs. 2010 Jan;34(1):46-54. doi: 10.1111/j.1525-1594.2009.00795.x. Epub 2009 Oct 11.
Osteogenic induction was regarded as an indispensable step for adipose-derived stromal cells (ADSCs) to have osteogenic ability. Non-induced ADSCs can also produce bone in vivo and heal skeletal defects. The present study aimed to compare the bone-forming ability of osteogenically induced ADSCs and non-induced ADSCs in vivo. Tissue-engineered constructs were prepared from osteogenically induced or non-induced ADSCs and porous hydroxyapatite/beta-tricalcium phosphate scaffolds. A scaffold without cells and an empty defect group were used as control. All were implanted in rat critical calvarial defects. After implantation for 6 and 12 weeks, bone formation was analyzed using histomorphometry and microcomputed tomography; there were no significant differences in the formation of new bone between osteogenically induced ADSCs and non-induced ADSCs (P > 0.05). In conclusion, osteogenic induction of ADSCs is not an indispensable step for bone formation in vivo. Non-induced ADSCs can also be used as seeding cells to construct bone tissue.
成骨诱导被认为是脂肪来源的基质细胞（ADSCs）具有成骨能力的不可或缺的步骤。未经诱导的 ADSCs 也可以在体内产生骨并修复骨缺损。本研究旨在比较体内诱导性 ADSCs 和非诱导性 ADSCs 的成骨能力。组织工程构建体由成骨性诱导的或未诱导的 ADSCs 和多孔羟基磷灰石/β-磷酸三钙支架制成。不含有细胞的支架和空缺陷组作为对照。所有的都被植入大鼠的临界颅骨缺损中。植入后 6 周和 12 周，使用组织形态计量学和微计算机断层扫描分析新骨形成; 成骨性诱导的 ADSCs 和非诱导性 ADSCs 之间的新骨形成没有显著差异（P>0.05）。总之，ADSCs 的成骨诱导不是体内骨形成的不可或缺的步骤。未经诱导的 ADSCs 也可用作种子细胞来构建骨组织。
