Department of Immunology, Stockholm University, Svante Arrheniusv 16, SE-106 91 Stockholm, Sweden.
Vaccine. 2009 Dec 10;28(1):90-7. doi: 10.1016/j.vaccine.2009.09.110. Epub 2009 Oct 12.
Antigen Pf332, a megadalton protein has been shown to be associated with the membrane of infected erythrocytes. Detailed functional studies on the antigen have remained hampered by the cross-reactive nature of antibodies generated to Pf332. Pf332-C231, identified in the C-terminal region of Pf332 was cloned and antibodies against the C231 fragment were shown to react with intact Pf332 antigen by both immunofluorescence and immunoblotting analyses. Antibodies to C231 inhibited in vitro Plasmodium falciparum growth efficiently. In addition, human sera from malaria-exposed individuals reacted with recombinant C231. We show that Pf332-C231 represents a functional domain and is expected to facilitate further studies on Pf332 as a potential target for protective immune responses and the function of the antigen.
抗原 Pf332 是一种与感染的红细胞膜相关的兆道尔顿蛋白。由于针对 Pf332 产生的抗体具有交叉反应性，因此对该抗原的详细功能研究一直受到阻碍。Pf332-C231 在 Pf332 的 C 末端区域被鉴定出来，并通过免疫荧光和免疫印迹分析显示针对 C231 片段的抗体与完整的 Pf332 抗原反应。针对 C231 的抗体可有效抑制体外疟原虫的生长。此外，来自疟疾暴露个体的人血清与重组 C231 反应。我们表明 Pf332-C231 代表一个功能域，有望促进进一步研究 Pf332 作为保护性免疫反应的潜在靶标和抗原的功能。
