淀粉样β蛋白/A4蛋白前体与阿尔茨海默病型痴呆中的神经原纤维缠结相关。
Amyloid beta/A4 protein precursor is bound to neurofibrillary tangles in Alzheimer-type dementia.
作者信息
Yamaguchi H, Ishiguro K, Shoji M, Yamazaki T, Nakazato Y, Ihara Y, Hirai S
机构信息
College of Medical Care and Technology, Gunma University, Japan.
出版信息
Brain Res. 1990 Dec 24;537(1-2):318-22. doi: 10.1016/0006-8993(90)90377-n.
Localization of amyloid beta/A4 protein precursor (APP) in Alzheimer's neurofibrillary tangles (NFT) was examined immunohistochemically. Antiserum directed to N-terminal of APP intensely labeled intracellular NFT and some neuropil threads. The NFT, extracted from Alzheimer brains by detergent treatments, were also immunoreactive with this antiserum. Antisera to other parts of APP labeled NFT after the formic acid pretreatment. However, Western blot analysis of NFT demonstrated no immunoreactive bands with APP antiserum. These findings suggest that APP is a minor component of the NFT.
采用免疫组织化学方法检测了淀粉样β/A4蛋白前体（APP）在阿尔茨海默病神经原纤维缠结（NFT）中的定位。针对APP N端的抗血清强烈标记细胞内NFT和一些神经毡丝。经去污剂处理从阿尔茨海默病大脑中提取的NFT也与该抗血清发生免疫反应。经甲酸预处理后，针对APP其他部位的抗血清标记了NFT。然而，NFT的蛋白质印迹分析显示与APP抗血清没有免疫反应条带。这些发现表明APP是NFT的次要成分。
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