从海葵卡里海葵中分离毒液的新方法。细胞溶解毒素的纯化与特性分析。
New method for isolation of venom from the sea anemone Actinia cari. Purification and characterization of cytolytic toxins.
作者信息
Sencic L, Macek P
机构信息
Department of Biology, Biotechnical Faculty, E. Kardelj University of Ljubljana, Yugoslavia.
出版信息
Comp Biochem Physiol B. 1990;97(4):687-93.
PMID:1982253
Abstract
- Venom from the sea anemone Actinia cari was obtained by the "milking" method. Two lethal and hemolytic polypeptide toxins, caritoxins I (CTX I) and II (CTX II), were isolated with gel and ion exchange chromatography. 2. The mol. wt of the pure toxin was 19,800. The isoelectric points of CTX I and II were 9.45 and 10.0, respectively. The toxins had similar amino acid compositions lacking cysteine. 3. The intravenous CTX I and CTX II lethal dose (LD50) in mice was found to be 54 +/- 25 and 90 +/- 1 micrograms/kg, respectively. Their hemolytic activity was inhibited by sphingomyelin.
摘要
- 采用“挤奶”法从海葵卡里海葵（Actinia cari）获取毒液。通过凝胶和离子交换色谱法分离出两种具有致死性和溶血性的多肽毒素，即卡里毒素I（CTX I）和II（CTX II）。2. 纯毒素的分子量为19,800。CTX I和II的等电点分别为9.45和10.0。这些毒素具有相似的氨基酸组成，不含半胱氨酸。3. 发现CTX I和CTX II对小鼠的静脉注射致死剂量（LD50）分别为54±25和90±1微克/千克。它们的溶血活性受到鞘磷脂的抑制。
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