Department of Molecular and Cellular Biology, Chang Gung University, Kwei-San, Tao-Yuan, 333, Taiwan.
Mol Biol Rep. 2010 Oct;37(7):3165-70. doi: 10.1007/s11033-009-9896-5. Epub 2009 Oct 13.
The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig(+) polA(+) strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA.
大肠杆菌中不连续 DNA 复制是否仅作用于滞后链，或者是否作用于两条链，这个问题尚未解决。在这项研究中，通过分析野生型、 lig-7 和 polA4113 遗传背景下合成的新生 DNA 的大小分布，研究了 priA、B、C 和 rep 基因在不连续 DNA 复制中的参与情况。priA 的失活，但不是 priB、priC 或 rep 的失活，导致 lig(+) polA(+) 野生型菌株中短脉冲标记的 DNA 中高分子量 (HMW) DNA 的显著增加。priA 的失活也导致 lig-7 和 polA4113 菌株中合成的新生 DNA 中 HMW DNA 的显著增加。这些结果表明 PriA 参与了新生 DNA 的不连续合成。
