Tang Jian-Jin, Wang Ming-Wei, Jia En-zhi, Yan Jian-Jun, Wang Qi-Ming, Zhu Jun, Yang Zhi-Jian, Lu Xiang, Wang Lian-sheng
Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, China.
Mol Biol Rep. 2010 Jan;37(1):405-10. doi: 10.1007/s11033-009-9877-8.
Recent studies suggest that the common variant in the GSTM1 and GSTT1 genes modifies the risk of coronary artery disease (CAD), however, it is unclear whether the risk of CAD modulated by variants in the GSTM1 and GSTT1 genes was associated with alterations of indices of oxidative stress and inflammation. Our study is an attempt to provide insight into the role of GST genetic variant and markers of oxidative stress and inflammation in CAD patients. A total of 719 Chinese CAD patients were successfully genotyped. Plasma total antioxidant status (TAOS), glutathione(GSH), C-reactive protein (CRP), fibrinogen (FIB) and white blood cell count (WBC) were determined to evaluate the oxidative stress and inflammatory response. The correlations between GSTM1/GSTT1 genotypes and alterations of indices of oxidative stress and inflammation were analyzed. We found GSTM1-0/GSTT1-0 subjects had higher CRP and FIB and lower TAOS compared to patients with wild-type GSTM1/GSTT1 genes. A stepwise elevations in age, the incidences of hypertension and diabetes mellitus, levels of FIB and the number of WBC were associated with increased number of stenosed vessels. Reductions of plasma TAOS and GSH were associated with increased number of stenosed vessels. Our results suggest that GST polymorphisms maybe modify the effect on markers of oxidative stress and inflammation in Chinese CAD patients.
近期研究表明，谷胱甘肽S转移酶M1（GSTM1）和谷胱甘肽S转移酶T1（GSTT1）基因的常见变异会改变冠状动脉疾病（CAD）的风险，然而，尚不清楚GSTM1和GSTT1基因变异所调节的CAD风险是否与氧化应激和炎症指标的改变相关。我们的研究旨在深入了解GST基因变异以及氧化应激和炎症标志物在CAD患者中的作用。总共对719名中国CAD患者成功进行了基因分型。测定血浆总抗氧化状态（TAOS）、谷胱甘肽（GSH）、C反应蛋白（CRP）、纤维蛋白原（FIB）和白细胞计数（WBC），以评估氧化应激和炎症反应。分析了GSTM1/GSTT1基因型与氧化应激和炎症指标改变之间的相关性。我们发现，与具有野生型GSTM1/GSTT1基因的患者相比，GSTM1-0/GSTT1-0受试者的CRP和FIB水平更高，而TAOS水平更低。年龄逐步升高、高血压和糖尿病的发病率、FIB水平以及WBC数量与狭窄血管数量增加相关。血浆TAOS和GSH的降低与狭窄血管数量增加相关。我们的结果表明，GST基因多态性可能会改变对中国CAD患者氧化应激和炎症标志物的影响。
