Division of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Dev Growth Differ. 2009 Dec;51(9):787-96. doi: 10.1111/j.1440-169X.2009.01137.x. Epub 2009 Oct 12.
The neural crest is a multipotent population of migratory cells that arises in the central nervous system and subsequently migrates along defined stereotypic pathways. In the present work, we analyzed the role of a repulsive axon guidance protein, draxin, in the migration of neural crest cells. Draxin is expressed in the roof plate of the chick trunk spinal cord and around the early migration pathway of neural crest cells. Draxin modulates chick neural crest cell migration in vitro by reducing the polarization of these cells. When exposed to draxin, the velocity of migrating neural crest cells was reduced, and the cells changed direction so frequently that the net migration distance was also reduced. Overexpression of draxin also caused some early migrating neural crest cells to change direction to the dorsolateral pathway in the chick trunk region, presumably due to draxin's inhibitory activity. These results demonstrate that draxin, an axon guidance protein, can also affect trunk neural crest migration in the chick embryo.
神经嵴是一种多能迁移细胞群体，起源于中枢神经系统，随后沿着特定的刻板途径迁移。在本工作中，我们分析了排斥性轴突导向蛋白 draxin 在神经嵴细胞迁移中的作用。Draxin 表达在鸡胚躯干脊髓的顶板和神经嵴细胞早期迁移途径周围。Draxin 通过减少这些细胞的极化来调节鸡神经嵴细胞的体外迁移。当暴露于 draxin 时，迁移的神经嵴细胞的速度降低，并且细胞经常改变方向，以至于净迁移距离也降低。Draxin 的过表达也导致一些早期迁移的神经嵴细胞向鸡胚躯干区域的背外侧途径改变方向，推测是由于 draxin 的抑制活性。这些结果表明，轴突导向蛋白 draxin 也可以影响鸡胚中的躯干神经嵴迁移。
