Zhang Sanbing, Cui Huixian, Wang Lei, Kang Lin, Huang Guannan, Du Juan, Li Sha, Tanaka Hideaki, Su Yuhong
Department of Hand and Foot Surgery, The Third Hospital of Shijiazhuang City, Shijiazhuang, People's Republic of China (SZ)
Department of Anatomy, Hebei Medical University, Shijiazhuang, Hebei Province, People's Republic of China (HC, LW, LK, JD, SL, YS)
J Histochem Cytochem. 2016 Jul;64(7):412-24. doi: 10.1369/0022155416646538. Epub 2016 May 19.
The appropriate projection of axons within the nervous system is a crucial component of the establishment of neural circuitry. Draxin is a repulsive axon guidance protein. Draxin has important functions in the guidance of three commissures in the central nervous system and in the migration of neural crest cells and dI3 interneurons in the chick spinal cord. Here, we report that the distribution of the draxin protein and the location of 23C10-positive areas have a strong temporal and spatial correlation. The overexpression of draxin, especially transmembrane draxin, caused 23C10-positive axon bundles to misproject in the dorsal hindbrain. In addition, the overexpression of transmembrane draxin caused abnormal formation of the ganglion crest of the IX and X cranial nerves, misprojection of some anti-human natural killer-1 (HNK-1)-stained structures in the dorsal roof of the hindbrain, and a simultaneous reduction in the efferent nerves of some motoneuron axons inside the hindbrain. Our data reveal that draxin might be involved in the fascicular projection of cranial nerves in the hindbrain.
轴突在神经系统内的正确投射是神经回路建立的关键组成部分。Draxin是一种轴突导向排斥蛋白。Draxin在中枢神经系统中三条连合的导向以及鸡脊髓中神经嵴细胞和dI3中间神经元的迁移中具有重要作用。在此，我们报道draxin蛋白的分布与23C10阳性区域的位置具有很强的时间和空间相关性。draxin的过表达，尤其是跨膜draxin的过表达，导致23C10阳性轴突束在后脑背侧错误投射。此外，跨膜draxin的过表达导致IX和X颅神经节嵴异常形成、后脑背顶中一些抗人自然杀伤细胞-1（HNK-1）染色结构的错误投射，以及后脑内一些运动神经元轴突的传出神经同时减少。我们的数据表明，draxin可能参与后脑颅神经的束状投射。
