Asano Y, Komuro T, Tada T
Department of Immunology, Faculty of Medicine, University of Tokyo, Japan.
Int Immunol. 1990;2(12):1203-11. doi: 10.1093/intimm/2.12.1203.
CD4 and CD8 T cell subsets involved in primary allo-MLR responses were re-evaluated in the present study. The slope analysis of B6 allo-MLR responses to MHC class I-disparate bm1 and class II-disparate bm12 revealed the presence of a T-T cell interaction between CD4 and CD8 T cell subsets. The allo-MLR response of B6 CD4 T cells to bm12 was completely blocked by anti-L3T4, indicating that the function of CD4 T cells is required for allo-MLR responses to bm12. It was further demonstrated that the function of CD8 T cells in class II-disparate allo-MLR is to augment the proliferative response of CD4 T cells. On the other hand, CD8 T cells primarily responded to class I-disparate allo-MLR, and CD4 T cells function to augment the proliferative response of CD8 T cells. It was further indicated that CD4 and CD8 T cells recognize both class I and class II MHC. The CD4 T cell recognition of allo-class I and CD8 T cell recognition of allo-class II induces insufficient signals for proliferation of these T cells. Thus, the class I-reactive CD4 and class II-reactive CD8 T cell subsets function as helper cells ('MLR helper' T cells) for proliferating T cells in MLR responses.
本研究对参与初次同种异体混合淋巴细胞反应（allo-MLR）的CD4和CD8 T细胞亚群进行了重新评估。B6对I类不匹配的bm1和II类不匹配的bm12的allo-MLR反应的斜率分析显示，CD4和CD8 T细胞亚群之间存在T-T细胞相互作用。B6 CD4 T细胞对bm12的allo-MLR反应被抗L3T4完全阻断，表明CD4 T细胞的功能对于对bm12的allo-MLR反应是必需的。进一步证明，在II类不匹配的allo-MLR中，CD8 T细胞的功能是增强CD4 T细胞的增殖反应。另一方面，CD8 T细胞主要对I类不匹配的allo-MLR作出反应，而CD4 T细胞的功能是增强CD8 T细胞的增殖反应。进一步表明，CD4和CD8 T细胞都识别I类和II类主要组织相容性复合体（MHC）。CD4 T细胞对同种I类的识别和CD8 T细胞对同种II类的识别诱导这些T细胞增殖的信号不足。因此，I类反应性CD4和II类反应性CD8 T细胞亚群在MLR反应中作为增殖T细胞的辅助细胞（“MLR辅助”T细胞）发挥作用。
