Yuan Jian-ye, Xie Jian-qun, Wu Da-zheng, Zheng Yu, Pan Xiang-xue, Fei Xiao-yan, Xu Hai-zhen
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Xi Yi Jie He Xue Bao. 2009 Oct;7(10):958-62. doi: 10.3736/jcim20091009.
To study the relationship between the inhibitory effects of Tongxie Yaofang, a compound traditional Chinese herbal medicine, on the contraction of the colonic smooth muscle isolated from rats and calcium mobilization.
By measuring the tension of the isolated colonic smooth muscle strips, the inhibitory effects of Tongxie Yaofang on the contraction induced by acetylcholine (ACh), KCl and exhausting Ca(2+) of internal calcium store were assessed respectively.
Tongxie Yaofang could concentration-dependently inhibit the contraction of isolated rat colonic smooth muscle strips induced by KCl and exhausting the Ca(2+) of internal calcium store. Tongxie Yaofang could also inhibit the tension of the second contractile phase induced by ACh (P<0.01, vs control), but had no influence on the first contractile phase.
Tongxie Yaofang can inhibit the contraction of isolated rat colonic smooth muscle strips mainly by preventing the influx of extracellular Ca(2+), which may be associated with blocking voltage-dependent channel, store-operated channel and receptor-operated channel, but not by preventing the release of internal Ca(2+) from calcium store.
研究中药复方痛泻要方对大鼠离体结肠平滑肌收缩及钙动员的抑制作用之间的关系。
通过测量离体结肠平滑肌条的张力，分别评估痛泻要方对乙酰胆碱（ACh）、氯化钾（KCl）诱导的收缩以及细胞内钙库钙耗尽诱导的收缩的抑制作用。
痛泻要方能够浓度依赖性地抑制KCl诱导的以及细胞内钙库钙耗尽诱导的大鼠离体结肠平滑肌条收缩。痛泻要方还能抑制ACh诱导的第二收缩相的张力（与对照组相比，P<0.01），但对第一收缩相无影响。
痛泻要方主要通过阻止细胞外Ca(2+)内流抑制大鼠离体结肠平滑肌条收缩，这可能与阻断电压依赖性通道、储存-操作性通道和受体-操作性通道有关，而不是通过阻止细胞内钙库释放Ca(2+)。
