Struijk D G, Koomen G C, Krediet R T, Arisz L
Department of Medicine, University of Amsterdam, The Netherlands.
Adv Perit Dial. 1990;6:289-91.
In vitro experiments were performed to analyze problems with the determination of erythropoietin in dialysate. Human recombinant erythropoietin (EPO; 4000 U/L) was added to several fluids, to glass or polystyrene tubes with or without addition of bovine serum albumin (BSA) and to dialysate bags. The recovery in peritoneal effluent was 4120 +/- 203 U/L (mean +/- SEM). The recovery in the other fluids was less than 50% but in glass tubes it increased to 96% after addition of BSA. Binding was also found to the dialysate bag, therefore reducing the amount available for absorption. We recommend that EPO samples from the dialysate are drawn within BSA coated glass tubes.
进行体外实验以分析透析液中促红细胞生成素测定的相关问题。将人重组促红细胞生成素（EPO；4000 U/L）添加到几种液体中、添加或不添加牛血清白蛋白（BSA）的玻璃或聚苯乙烯管中以及透析袋中。腹腔流出液中的回收率为4120±203 U/L（平均值±标准误）。其他液体中的回收率低于50%，但在玻璃管中添加BSA后回收率增至96%。还发现透析袋存在结合现象，从而减少了可供吸收的量。我们建议在涂有BSA的玻璃管内采集透析液中的EPO样本。
