Paniagua R, Martinez-Onsurbe P, Santamaria L, Saez F J, Amat P, Nistal M
Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
Int J Androl. 1990 Dec;13(6):470-87. doi: 10.1111/j.1365-2605.1990.tb01054.x.
A quantitative and ultrastructural study was performed on biopsies of human cryptorchid testes to investigate lesions in the lamina propria and Sertoli cells. Prepubertal cryptorchid testes (1-9 years of age) were classified into four groups: Type 1, testes with minimal lesions; Type II, testes with a moderate decrease in tubule diameter and spermatogonal number; Type III, testes with Sertoli cell hypoplasia and a marked reduction in tubule diameter and spermatogonal number; and Type IV, testes with Sertoli cell hyperplasia and a variable reduction in spermatogonal number. An increase in thickness of the lamina propria was found in Type II and III testes from 5 years of age onwards. These testes also showed a decrease in both the average number of peritubular cells per cross-sectioned tubule and in the average nuclear volume of these cells. Most of the postpubertal cryptorchid testes from 13- to 18-year-old youths presented a prepubertal pattern suggestive of delayed testicular maturation. Postpubertal testes from 19- to 27-year-old men were classified into three types: Type A testes showed complete spermatogenesis, mature Sertoli cells and no lesions in the lamina propria; Type B testes showed isolated spermatogonia, mature Sertoli cells, and a marked thickening of the lamina propria; and Type C testes showed isolated spermatogonia, hyperplasia of immature Sertoli cells, and a slightly thickened lamina propria. Maturation of the lamina propria was always associated with maturation of the Sertoli cells. Thickening of the lamina propria was associated with peritubular cell alterations consisting of decreases in the nuclear volume (average and total per testis) of peritubular cells and increases in the number of these cells per cross-sectioned tubule. The three types of adult cryptorchid testes appear to be the postpubertal transformation of Type 1 testes (Type A), Type II and Type III testes (Type B), and Type IV testes (Type C).
对人类隐睾活检组织进行了定量和超微结构研究，以调查固有层和支持细胞的病变情况。青春期前隐睾（1 - 9岁）分为四组：1型，病变轻微的睾丸；2型，小管直径和精原细胞数量中度减少的睾丸；3型，支持细胞发育不全且小管直径和精原细胞数量显著减少的睾丸；4型，支持细胞增生且精原细胞数量可变减少的睾丸。从5岁起，在2型和3型睾丸中发现固有层厚度增加。这些睾丸还显示，每个横切小管的管周细胞平均数量以及这些细胞的平均核体积均减少。大多数13至18岁青少年的青春期后隐睾呈现出青春期前模式，提示睾丸成熟延迟。19至27岁男性的青春期后睾丸分为三种类型：A型睾丸显示完全精子发生、成熟的支持细胞且固有层无病变；B型睾丸显示孤立的精原细胞、成熟的支持细胞以及固有层明显增厚；C型睾丸显示孤立的精原细胞、未成熟支持细胞增生以及固有层轻度增厚。固有层的成熟总是与支持细胞的成熟相关。固有层增厚与管周细胞改变有关，表现为管周细胞核体积（平均和每个睾丸的总体积）减少以及每个横切小管中这些细胞数量增加。三种类型的成人隐睾似乎分别是1型睾丸（A型）、2型和3型睾丸（B型）以及4型睾丸（C型）的青春期后转变。
