机械应力对细胞黏附的影响：形态变化的一种可能机制。
Effects of mechanical stress on cell adhesion: a possible mechanism for morphological changes.
机构信息
Department of Clinical and Experimental Medicine, Human Anatomy, University of Eastern Piedmont, Novara, Italy.
出版信息
Cell Adh Migr. 2010 Jan-Mar;4(1):19-25. doi: 10.4161/cam.4.1.9569. Epub 2010 Jan 21.
Abstract
The transmission of mechanical forces to cells is followed among all by biological signals related to changes in the assembly or disassembly of integrins associated linker proteins, such as vinculin. We applied for 3 hours 2% cyclic mechanical strain at the frequency of 1 Hz to human fibroblasts cultured on a deformable substrate; substrate deformation resulted to modify the number, length and area of vinculin positive focal adhesion contacts when compared to not stretched cells. The mechanism behind these morphological changes is related to Akt and RhoA roles in focal adhesion assembly. In the case of Akt and Rho inhibition, focal contacts disassembled only in presence of stretching mechanical stress, highlighting the role of mechanical stress on focal adhesion maturation in terms of multimolecolar assembly which from focal complexes leads to fibrillar adhesion.
摘要
机械力向细胞的传递首先涉及到与整合素相关连接蛋白（如 vinculin）的组装或拆卸变化相关的生物信号。我们将 2%的周期性机械应变以 1Hz 的频率施加于在可变形基底上培养的人成纤维细胞 3 小时；与未拉伸细胞相比，基底变形导致 vinculin 阳性黏附斑接触的数量、长度和面积发生改变。这些形态变化的背后机制与 Akt 和 RhoA 在黏附斑组装中的作用有关。在 Akt 和 Rho 抑制的情况下，只有在存在拉伸机械应力的情况下黏附斑才会解体，这突出了机械应力在黏附斑成熟方面的作用，涉及到从黏附斑复合物到纤维状黏附的多分子组装。
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