Panganiban G E, Reuter R, Scott M P, Hoffmann F M
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Development. 1990 Dec;110(4):1041-50. doi: 10.1242/dev.110.4.1041.
The decapentaplegic (dpp) gene product, a member of the transforming growth factor-beta family, is required in Drosophila embryos for normal gastrulation and the establishment of dorsal-ventral polarity in the embryo. dpp is also expressed at specific positions in the visceral mesoderm along the developing midgut. We find that mutations that eliminate the visceral mesoderm expression of dpp lead to defects in midgut morphogenesis and alter the spatially localized expression of the homeotic genes Sex combs reduced (Scr), Ultrabithorax (Ubx), and Antennapedia (Antp) in the visceral mesoderm. The extracellular dpp protein migrates from the visceral mesoderm across the apposing endodermal cell layer in a region of the endoderm that expresses the homeotic gene labial (lab). Mesodermal expression of dpp is required for the expression of lab in these endodermal cells indicating that dpp mediates an inductive interaction between the two germ layers. We propose that extracellular dpp protein regulates gut morphogenesis, in part, by regulating homeotic gene expression in the visceral mesoderm and endoderm of the developing midgut.
截瘫（dpp）基因产物是转化生长因子-β家族的成员之一，在果蝇胚胎中，它对于正常的原肠胚形成以及胚胎背腹极性的建立是必需的。dpp也在内脏中胚层沿着发育中的中肠的特定位置表达。我们发现，消除dpp在内脏中胚层表达的突变会导致中肠形态发生缺陷，并改变同源异型基因性梳减少（Scr）、超双胸（Ubx）和触角足（Antp）在内脏中胚层的空间定位表达。细胞外的dpp蛋白从内脏中胚层穿过在内胚层一个表达同源异型基因唇（lab）的区域中相对的内胚层细胞层迁移。dpp在内脏中胚层的表达是这些内胚层细胞中lab表达所必需的，这表明dpp介导了两个胚层之间的诱导相互作用。我们提出，细胞外dpp蛋白部分地通过调节发育中肠的内脏中胚层和内胚层中的同源异型基因表达来调控肠道形态发生。
