Leal L M, Dosreis G A
Instituto Nacional do Câncer (INCa), Universidade Federal do Rio de Janeiro, Brasil.
Braz J Med Biol Res. 1990;23(9):835-9.
Normal resting spleen T lymphocytes from mice were stimulated in vitro by monoclonal antibodies (mAbs) against either Thy 1 or CD3:TcR surface protein molecules. Although both mAbs were mitogenic, anti-Thy 1 activation generated 5 times more IL2 secretion than anti-CD3 activation under similar conditions. Production of IL3-like activity was comparable for both Thy1 and CD3-mediated activation. In addition, non-mitogenic doses of anti-CD3 and anti-Thy1 (0.16 micrograms/ml and 0.0125% ascites, respectively) mAbs induced T cell activation when provided together. These results indicate that Thy1 signalling cooperates with the CD3:TcR pathway to activate T cells. However, the Thy1 pathway is also regulated independently since IL2 production is larger when stimulated by anti-Thy1 than anti-CD3 mAbs.
来自小鼠的正常静息脾T淋巴细胞在体外被抗Thy 1或抗CD3：TcR表面蛋白分子的单克隆抗体（mAb）刺激。尽管两种单克隆抗体都具有促有丝分裂作用，但在相似条件下，抗Thy 1激活产生的IL2分泌量比抗CD3激活多5倍。Thy1和CD3介导的激活所产生的IL3样活性相当。此外，非促有丝分裂剂量的抗CD3和抗Thy1单克隆抗体（分别为0.16微克/毫升和0.0125%腹水）一起提供时可诱导T细胞激活。这些结果表明，Thy1信号传导与CD3：TcR途径协同激活T细胞。然而，Thy1途径也是独立调节的，因为抗Thy1单克隆抗体刺激时IL2的产生量比抗CD3单克隆抗体刺激时更大。
