Weissman B E
Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27516.
Cancer Surv. 1990;9(3):475-85.
The technique of somatic cell hybridization and the concept of tumour suppressor genes share a common history. Somatic cell hybrids between mammalian tumour cells and their normal cellular counterparts invariably are non-tumorigenic, establishing the recessive genetic nature of tumorigenicity. The development of cytogenetics and microcell hybridization has provided methods for localization of the tumour suppressor genes to specific chromosomal regions. Continuing studies of this type will facilitate the isolation of these genes by molecular methods including differential cDNA screening and 'reverse genetics'.
体细胞杂交技术与肿瘤抑制基因的概念有着共同的历史渊源。哺乳动物肿瘤细胞与其正常细胞对应物之间的体细胞杂种始终是非致瘤性的，这确立了致瘤性的隐性遗传本质。细胞遗传学和微细胞杂交技术的发展为将肿瘤抑制基因定位到特定染色体区域提供了方法。这类持续的研究将有助于通过包括差异cDNA筛选和“反向遗传学”在内的分子方法分离这些基因。
