Gupta D K, Lemansky P A, Tartakoff A M
Department of Pathology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106.
Indian J Biochem Biophys. 1990 Dec;27(6):416-9.
Thy-1 is a major glycophospholipid (GPL)-anchored protein found on the surface of neurons, epithelial cells, fibroblasts and murine T-lymphomas. Biochemical studies were undertaken to determine if murine T-lymphomas contain glycolipids which may be on the path of GPL-anchor biosynthesis. Biosynthetic labeling experiments on Thy-1-positive (wild-type) cells followed by battery of chemical and enzymatic diagnostics on the isolated [3H]mannolipids have, for the first time, led to description of a set of glycolipids which have properties consistent with their being GPL-anchor precursors. Using these results as a guide, major differences have been observed upon analysis of the radiolabeled mannolipids of Thy-1-negative mutants from 7 complementation classes, A-C, E, F, H and I. The biosynthetic lesions in anchor synthesis have been identified in some of the mutants.
Thy-1是一种主要的糖磷脂（GPL）锚定蛋白，存在于神经元、上皮细胞、成纤维细胞和鼠类T淋巴瘤的表面。开展了生化研究，以确定鼠类T淋巴瘤是否含有可能处于GPL锚生物合成途径中的糖脂。对Thy-1阳性（野生型）细胞进行生物合成标记实验，随后对分离出的[3H]甘露糖脂进行一系列化学和酶诊断，首次描述了一组具有与GPL锚前体一致特性的糖脂。以这些结果为指导，在分析来自7个互补类（A - C、E、F、H和I）的Thy-1阴性突变体的放射性标记甘露糖脂时，观察到了主要差异。在一些突变体中已确定了锚定合成中的生物合成损伤。
