Lemansky P, Gupta D K, Meyale S, Tucker G, Tartakoff A M
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Mol Cell Biol. 1991 Aug;11(8):3879-85. doi: 10.1128/mcb.11.8.3879-3885.1991.
Essentially all eukaryotic cells, including murine lymphomas, express surface proteins, such as Thy-1, which are anchored by a phosphoinositol mannolipid. Putative mannolipid anchor precursors can be detected in these cells. Six distinct Thy-1-negative lymphoma mutants lack complete mannolipids, and three mutants synthesize atypical mannolipids. The absence of complete mannolipids can account for the lack of expression of multiple mannolipid-anchored proteins and may also account for the lack of lipid anchoring in the human disease paroxysmal nocturnal hemoglobinuria. Structural information on the mannolipids of wild-type and mutant cells indicates that anchor biosynthesis in these cells may involve both transmembrane flip-flop of intermediates and a deacylation step.
基本上所有真核细胞，包括鼠淋巴瘤细胞，都表达表面蛋白，如Thy-1，这些蛋白由磷酸肌醇甘露糖脂锚定。在这些细胞中可以检测到假定的甘露糖脂锚定前体。六个不同的Thy-1阴性淋巴瘤突变体缺乏完整的甘露糖脂，三个突变体合成非典型甘露糖脂。完整甘露糖脂的缺失可以解释多种甘露糖脂锚定蛋白表达的缺乏，也可能解释人类疾病阵发性夜间血红蛋白尿中脂质锚定的缺乏。野生型和突变体细胞甘露糖脂的结构信息表明，这些细胞中的锚定生物合成可能涉及中间体的跨膜翻转和脱酰基步骤。
