Institut für Energie- und Umwelttechnik (IUTA) e. V., Bliersheimer Str. 60, D-47229 Duisburg, Germany.
J Chromatogr A. 2009 Nov 27;1216(48):8470-9. doi: 10.1016/j.chroma.2009.09.075. Epub 2009 Oct 4.
This paper is the second in a series of consecutive publications, explaining the concept of high temperature liquid chromatography under various important aspects. The second publication deals with the determination of the viscosity of binary solvent mixtures used in reversed phase liquid chromatography in a temperature range between 25 and 250 degrees C. In literature, only limited data of the temperature dependent viscosities of liquid solvents or binary solvent mixtures can be found. Therefore, the viscosities of the pure solvents as well as the binary mixtures had to be determined experimentally up to 250 degrees C. The viscosity data were used to estimate the pressure drop in a capillary connecting a high-temperature HPLC system with a mass spectrometer. The solvent perturbation could be avoided by adjusting the diameter of the transfer capillary to the viscosity and vapour pressure of the mobile phase. The viscosity data were also used to show that a significant gain in analysis speed is theoretically feasible. This factor clearly depends on the nature of the solvent system, because for mixtures with a large viscosity maximum at ambient temperature, this effect is most pronounced.
本文是一系列连续出版物中的第二篇，从各个重要方面解释高温液相色谱的概念。第二篇涉及在 25 至 250°C 的温度范围内测定反相液相色谱中使用的二元溶剂混合物的粘度。在文献中，只能找到关于液体溶剂或二元溶剂混合物的温度依赖性粘度的有限数据。因此，必须在 250°C 以下实验测定纯溶剂以及二元混合物的粘度。使用粘度数据来估算将高温 HPLC 系统与质谱仪连接的毛细管中的压降。通过将传输毛细管的直径调整到流动相的粘度和蒸气压，可以避免溶剂干扰。粘度数据还用于表明在理论上可以实现显著的分析速度提高。该因素显然取决于溶剂系统的性质，因为对于在环境温度下具有最大粘度的混合物，这种效果最为明显。
