Zhao Liang, Fan Li, Zhang Xu, Tan Wensong
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Jul;25(7):1069-76.
As an immunodepressant, anti-CD25 monoclonal antibody has a huge market with wide prospect and economic value. We developed a low protein serum-free medium for large-scale GS-NS0 myeloma cell culture and anti-CD25 monoclonal antibody production. Further study focused on the characteristics of GS-NSO cell growth, glucose and amino acid metabolism, and antibody production. In the serum-free medium, the maximal viable cell density and antibody concentration reached above 3x10(6) cells/mL and 300 mg/L in batch culture. Compared with the commercial serum-free medium (Excell 620 + 0.2% Primatone), the maximal viable cell density doubled and the maximal antibody concentration increased 46%. Results also showed the specific growth rate decreased when the glucose concentration was lower than 6 mmol/L. And the production of lactate increased when glucose concentration was excessively high (> 30 mmol/L). These results were important to provide technique and theory basis for developing optimized GS-NS0 cell culture and anti-CD25 monoclonal antibody production processes.
作为一种免疫抑制剂，抗CD25单克隆抗体具有巨大的市场，前景广阔且具有经济价值。我们开发了一种用于大规模GS-NS0骨髓瘤细胞培养和抗CD25单克隆抗体生产的低蛋白无血清培养基。进一步的研究集中在GS-NS0细胞生长特性、葡萄糖和氨基酸代谢以及抗体生产方面。在无血清培养基中，分批培养时最大活细胞密度和抗体浓度分别达到3×10(6)个细胞/mL以上和300 mg/L以上。与市售无血清培养基（Excell 620 + 0.2% Primatone）相比，最大活细胞密度翻倍，最大抗体浓度提高了46%。结果还表明，当葡萄糖浓度低于6 mmol/L时，比生长速率下降。当葡萄糖浓度过高（> 30 mmol/L）时，乳酸产量增加。这些结果对于开发优化的GS-NS0细胞培养和抗CD25单克隆抗体生产工艺提供技术和理论依据具有重要意义。
