Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, viale Annunziata, 98168 Messina, Italy.
J Chromatogr A. 2010 Feb 12;1217(7):1101-5. doi: 10.1016/j.chroma.2009.09.080. Epub 2009 Oct 4.
The present research is focused on the determination of the enantiomeric distribution of chiral compounds, contained in mandarin essential oils, by means of conventional chiral gas chromatography with flame ionization detection (enantio-GC-FID); the results attained were compared with those derived from heart-cutting multidimensional GC-mass spectrometry (MDGC/MS), to evaluate the reliability of the monodimensional technique as a tool for quality control. The Deans-switch MDGC system was equipped with two GC ovens, which were connected via a heated transfer line, a flame ionization detector (FID1) in the first dimension and a quadrupole MS as second-dimension detector. The a priori knowledge of potential co-elutions concerning target compounds (an enantiomer and an interfering compound), when using enantio-GC-FID, could enable the use of corrected enantiomer excess values. Correction factors could be calculated through a preliminary GC-FID analysis (using an apolar column), considering the peak areas of the known interferences. The method used for the calculation of a so-called "coelution correction factor" is described, along with some examples.
本研究旨在通过常规手性气相色谱-火焰离子化检测（对映异构体气相色谱-火焰离子化检测）来确定包含在柑橘精油中的手性化合物的对映体分布；将所得结果与来自分段多维气相色谱-质谱（MDGC/MS）的结果进行比较，以评估单维技术作为质量控制工具的可靠性。Dean 切换多维气相色谱系统配备了两个 GC 炉，通过加热传输线连接，在第一维使用火焰离子化检测器（FID1），在第二维使用四极杆 MS 作为检测器。在使用对映异构体气相色谱-火焰离子化检测时，有关目标化合物（对映异构体和干扰化合物）潜在共洗脱的先验知识可以使使用校正的对映体过量值成为可能。校正因子可以通过初步的 GC-FID 分析（使用非极性柱）来计算，同时考虑已知干扰物的峰面积。本文描述了一种计算所谓“共洗脱校正因子”的方法，并提供了一些示例。
