α粒子对斑马鱼胚胎的影响。

Effects of alpha particles on zebrafish embryos.

作者信息

Yum E H W, Li V W T, Choi V W Y, Cheng S H, Yu K N

机构信息

Department of Physics and Materials Science, City University of Hong Kong, Kowloon Tong, Hong Kong.

出版信息

Appl Radiat Isot. 2010 Apr-May;68(4-5):714-7. doi: 10.1016/j.apradiso.2009.09.035. Epub 2009 Sep 19.

DOI:10.1016/j.apradiso.2009.09.035

PMID:19837598

Abstract

Dechorionated zebrafish embryos were irradiated at 1.5 h post fertilization (hpf) to low-doses of alpha particles, viz., 1.4, 2.8, 5.6, 11.2 mGy (determined using Monte Carlo simulations). At 24 hpf, these embryos were then examined for apoptotic cells through acridine orange staining. The mean number of apoptotic cells was found to decrease significantly from controls to 1.4-mGy irradiation, and then to increase almost linearly to 2.8, 5.6 and 11.2-mGy irradiation. This trend is a typical characteristic of a hormetic effect.

摘要

在受精后1.5小时（hpf），去除绒毛膜的斑马鱼胚胎接受低剂量的α粒子照射，即1.4、2.8、5.6、11.2毫戈瑞（使用蒙特卡洛模拟确定）。在24 hpf时，通过吖啶橙染色检查这些胚胎中的凋亡细胞。发现凋亡细胞的平均数量从对照组到1.4毫戈瑞照射时显著减少，然后几乎呈线性增加到2.8、5.6和11.2毫戈瑞照射。这种趋势是兴奋效应的典型特征。