Riley R L, Kruger M G, Landa B, Elia J, Ringel A
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33101.
Clin Immunol Immunopathol. 1991 Jan;58(1):33-45. doi: 10.1016/0090-1229(91)90146-2.
Humoral immune responses of (NZB x NZW)F1 (BWF1) autoimmune mice to T cell-dependent antigens often exhibit a predominance of IgG2 antibodies, while normal mice produce IgG1 antibodies. In order to determine whether this results from differences in properties of the B cells or the T cells involved, the responses of both primary and secondary BWF1 B cells to the antigen DNP-hemocyanin (Hy) were measured in limiting dilution splenic fragment cultures in the presence of normal T cell help. Furthermore, the capacity of Hy-primed lymph node T cells from BWF1 mice to provide help to BALB/c nu/nu B cells was determined in modified splenic fragment cultures. These experiments indicated that (a) stimulation of primary BWF1 B cells with DNP-Hy and normal T cell help failed to yield significant numbers of clones which produced any of the IgG isotypes; (b) antigenic stimulation of BWF1 secondary B cell clones also demonstrated a paucity of IgG1, but elevated production of IgG2 isotypes; and (c) Hy-primed BWF1 lymph node T cells were comparable to those derived from BALB/c mice in their capacity to provide both help for nu/nu B cell responses and modulation of IgG isotype switching. BWF1 B cells apparently differ from normal murine B cells in their capacity to produce IgG antibodies upon T cell-dependent antigenic stimulation.
（新西兰黑鼠×新西兰白鼠）F1（BWF1）自身免疫小鼠对T细胞依赖性抗原的体液免疫反应通常以IgG2抗体为主，而正常小鼠则产生IgG1抗体。为了确定这是由所涉及的B细胞还是T细胞的特性差异所致，在正常T细胞辅助存在的情况下，通过有限稀释脾细胞片段培养法测定了初级和次级BWF1 B细胞对抗原二硝基苯基血蓝蛋白（Hy）的反应。此外，在改良的脾细胞片段培养中，测定了来自BWF1小鼠的经Hy致敏的淋巴结T细胞向BALB/c裸鼠B细胞提供辅助的能力。这些实验表明：（a）用DNP-Hy和正常T细胞辅助刺激初级BWF1 B细胞未能产生大量产生任何IgG同种型的克隆；（b）对BWF1次级B细胞克隆的抗原刺激也显示IgG1缺乏，但IgG2同种型的产生增加；（c）经Hy致敏的BWF1淋巴结T细胞在为裸鼠B细胞反应提供辅助以及调节IgG同种型转换的能力方面与来自BALB/c小鼠的T细胞相当。在T细胞依赖性抗原刺激下，BWF1 B细胞产生IgG抗体的能力显然不同于正常小鼠B细胞。
