Ma Jie, Cao Ying, Hu Jian-Li, Sun Hui, Liu Lin-Xiang, Zhao Chun-Hua
Department of Hematology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Oct;17(5):1289-93.
The aim of this study was to evaluate the therapeutic potential of bone marrow mesenchymal stem cells (MSC) on acute liver injury induced by concanavalin A (ConA). MSCs were isolated from male C57BL/6 mice and cultured, and a ConA-induced acute liver injury model was used. MSCs were systemically infused immediately after mice were challenged with ConA, control mice received only saline infusion. 24 hours after MSC transplantation, the level of serum aminotransferases, histologic change and in situ apoptosis of cells were detected, the expression of inflammatory mediators were examined by real-time RT-PCR. The results indicated that MSC transplantation significantly reduced ConA-induced acute liver injury, including the decrease of the level of serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST) and the extenuation of liver necrosis and in situ apoptosis. Furthermore, after MSC infusion the expression of TNF-alpha, IFN-gamma in liver decreased greatly (p<0.05) with no statistical difference in the expression of iNOS, IL-2 and IL-10 (p>0.05). It is concluded that the systemic infusion of MSCs can alleviate ConA induced acute liver injury in mice.
本研究旨在评估骨髓间充质干细胞（MSC）对刀豆蛋白A（ConA）诱导的急性肝损伤的治疗潜力。从雄性C57BL/6小鼠中分离并培养MSC，采用ConA诱导的急性肝损伤模型。在用ConA攻击小鼠后立即全身输注MSC，对照小鼠仅输注生理盐水。MSC移植24小时后，检测血清转氨酶水平、组织学变化和细胞原位凋亡情况，通过实时RT-PCR检测炎症介质的表达。结果表明，MSC移植显著减轻了ConA诱导的急性肝损伤，包括血清丙氨酸转氨酶（ALT）水平、血清天冬氨酸转氨酶（AST）水平的降低以及肝坏死和原位凋亡的减轻。此外，输注MSC后，肝脏中TNF-α、IFN-γ的表达显著降低（p<0.05），而iNOS、IL-2和IL-10的表达无统计学差异（p>0.05）。结论是，全身输注MSC可减轻ConA诱导的小鼠急性肝损伤。
