Chen Lin-Feng, Liu Jing-Han, Ouyang Xi-Lin, Zhuang Yuan, Che Ji, Yu Yang
Department of Transfusion, Chinese PLA General Hospital, Beijing 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Oct;17(5):1368-72.
This study was purposed to investigate the effect of different compositions and concentrations of lyophilizing protectants on recovery of RBCs and hemoglobin (Hb) after rehydration of lyophilized RBCs. The RBC lyophilizing protectants composed of a series concentrations of PVP, trehalose and different osmotic protectants were applied for protecting lyophilizing process of RBCs, the recovery of RBCs and Hb after rehydration of lyophilized RBCs was detected. The results showed that there were significant differences in loss ratio of RBCs between protectants composed of different compositions and concentrations (p<0.05 or p<0.01). The loss ratio of RBCs in protectant containing 30% PVP40, 150 mmol/L trehalose and 2% BSA was minimum (0.02%), the loss ratio of RBCs in protectant containing 6% PVP 360, 100 mmol/L trehalose and 2% BSA was maximum (0.27%). The difference of effect between 150 and 50 mmol/L trehalose was statistically significant (p<0.01). The recovery rates of RBCs and Hb in protectants contained PVP40 of different concentrations were different after rehydration of lyophilized RBCs. The protectant containing 15% PVP40, 150 mmol/L trehalose and 2% BSA showed optimal protective efficacy for lyophilized RBCs, the recovery rates of RBCs and Hb were 61.29+/-4.11% and 62.49+/-5.91% respectively, which were statistically different from other protectants (p<0.01). The protectants containing glycerol displayed best efficiency in lyophilization too, the recovery rates of RBCs and Hb were 65.97+/-4.52% and 67.24+/-5.94%, respectively. It is concluded that the protectants composed of 0.8 mol/L glycerol, 15% PVP40, 150 mmol/L trehalose and 2% BSA (pH 7.3 ) may be used as the protectant lyophilizing human RBCs in future study.
本研究旨在探讨不同组成和浓度的冻干保护剂对冻干红细胞复水后红细胞（RBC）及血红蛋白（Hb）恢复情况的影响。应用由一系列浓度的聚乙烯吡咯烷酮（PVP）、海藻糖及不同渗透保护剂组成的红细胞冻干保护剂对红细胞冻干过程进行保护，并检测冻干红细胞复水后RBC及Hb的恢复情况。结果显示，不同组成和浓度的保护剂之间RBC损失率存在显著差异（p<0.05或p<0.01）。含30% PVP40、150 mmol/L海藻糖和2%牛血清白蛋白（BSA）的保护剂中RBC损失率最低（0.02%），含6% PVP 360、100 mmol/L海藻糖和2% BSA的保护剂中RBC损失率最高（0.27%）。150 mmol/L与50 mmol/L海藻糖之间的效果差异具有统计学意义（p<0.01）。冻干红细胞复水后，不同浓度PVP40的保护剂中RBC及Hb的恢复率不同。含15% PVP40、150 mmol/L海藻糖和2% BSA的保护剂对冻干红细胞显示出最佳保护效果，RBC及Hb的恢复率分别为61.29±4.11%和62.49±5.91%，与其他保护剂相比有统计学差异（p<0.01）。含甘油的保护剂在冻干中也显示出最佳效果，RBC及Hb的恢复率分别为65.97±4.52%和67.24±5.94%。结论：含0.8 mol/L甘油、15% PVP40、150 mmol/L海藻糖和2% BSA（pH 7.3）的保护剂可能在未来研究中用作冻干人红细胞的保护剂。
