Fan Xing, Liao Sha, Sha Li-Na, Liu Jing, Wang Xiao-Li, Zhou Yong-Hong
Triticeae Research Institute, Chengdu Academy of Sciences, Sichuan Agricultural University, Wenjiang 611130, China.
Yi Chuan. 2009 Oct;31(10):1049-58. doi: 10.3724/sp.j.1005.2009.01049.
To estimate the phylogenetic relationships between Hystrix and its related genera (Poaceae: Triticeae), the sequences of the single-copy nuclear gene Pgk1 were analyzed for five Hystrix taxa, together with three Pseudoroegneria (St) species, two Hordeum (H) species, two Psathyrostachys (Ns) species, four Elymus (StH) species, five Leymus (NsXm) species, Thinopyrum bessarabicum (E(b)), and Lophopyrum elongatum (E(e)). Sequence analysis indicated that an 81bp Stowaway insertion occurred in the Pgk1 sequences of L. arenarius and Psa. juncea, and a 29 bp Copia insertion occurred in the Pgk1 sequences of Hy. duthiei, Hy. duthiei ssp. longearistata and L. akmolinensis. Phylogenetic analysis indicated that: (1) Hy. patula was closely related to Pseudoroegneria, Hordeum, and Elymus; (2) Hy. duthiei, Hy. duthiei ssp. longearistata, Hy. coreana, and Hy. komarovii were closely related to Psathyrostachys and Leymus. Based on these results, it is reasonable to transfer Hy. patula from Hystrix to Elymus, and to combine Hy. duthiei, Hy. duthiei ssp. longearistata, Hy. coreana, and Hy. komarovii into Leymus.
为了估计猬草属及其近缘属(禾本科:小麦族)之间的系统发育关系,对5个猬草属分类群的单拷贝核基因Pgk1序列进行了分析,并与3个拟鹅观草属(St)物种、2个大麦属(H)物种、2个新麦草属(Ns)物种、4个披碱草属(StH)物种、5个赖草属(NsXm)物种、贝加尔针茅(E(b))和长穗偃麦草(E(e))一起分析。序列分析表明,在沙生赖草和新麦草的Pgk1序列中发生了一个81bp的Stowaway插入,在钩刺猬草、长芒猬草和阿克莫林赖草的Pgk1序列中发生了一个29bp的Copia插入。系统发育分析表明:(1)扁穗猬草与拟鹅观草属、大麦属和披碱草属关系密切;(2)钩刺猬草、长芒猬草、朝鲜猬草和 Komarov猬草与新麦草属和赖草属关系密切。基于这些结果,将扁穗猬草从猬草属转移到披碱草属,并将钩刺猬草、长芒猬草、朝鲜猬草和Komarov猬草合并到赖草属是合理的。