Bua Alessandra, Rosu Valentina, Molicotti Paola, Das Gupta Sujoy K, Ahmed Niyaz, Zanetti Stefania, Sechi Leonardo A
Dipartimento di Scienze Biomediche, Università di Sassari, Sassari, Italy.
New Microbiol. 2009 Jul;32(3):293-6.
This study evaluated the possibility to use six phages specific to the Mycobacterium tuberculosis lipoarabinomannan (LAM) as tools for tubercular serodiagnosis. We analysed sera samples from 30 subjects with active tuberculosis (TB+), 30 with latent tubercular infection (LTBI) and 60 healthy subjects as controls (K). Our data indicated a good antibody response of the TB+ and LTBI patients against the phage Ri(7)17; the optical density (OD) values obtained from sera patients was statistically significant when compared to the control samples. Our results confirm that phage display technology might be useful to develop new tools for diagnosis of tuberculosis.
本研究评估了使用六种针对结核分枝杆菌脂阿拉伯甘露聚糖（LAM）的噬菌体作为结核病血清学诊断工具的可能性。我们分析了30例活动性结核病患者（TB+）、30例潜伏性结核感染（LTBI）患者的血清样本以及60例健康对照者（K）的血清样本。我们的数据表明，TB+和LTBI患者对噬菌体Ri(7)17有良好的抗体反应；与对照样本相比，患者血清获得的光密度（OD）值具有统计学意义。我们的结果证实，噬菌体展示技术可能有助于开发用于结核病诊断的新工具。
