Tang Tao, Muneta Takeshi, Sekiya Ichiro
Section of Orthopedic Surgery, Graduate School, Tokyo Medical and Dental University, Japan.
J Med Dent Sci. 2008 Mar;55(1):163-73.
The objective of the study was to determine whether strenuous running exercise produces a significant amount of fibrosis in the infrapatellar fat pad (IFP) of the knee and whether intraarticular injections of hyaluronan (HA) protect fibrosis in the IFP in a rat running model. Strenuous running exercise was performed on a rodent treadmill with 5% inclination using Wistar rats. After the exercise, hyaluronan was injected into the right knee joint once per week in the HA injection group. In the treatment control group of the left knee, no injection was done. Also, the running exercise group and the sedentary control group received no HA injection. The IFP was analyzed histologically and immunohistochemically after 1 week (5 km), 2 weeks (10 km), 3 weeks (15 km) and 6 weeks (30 km). The inflammatory reaction in the IFP was not prominent at each running stage. A slightly, but significantly increased amount of inflammatory cell infiltration appeared during strenuous running. The amount of collagen fibers increased significantly at 30 km stage. Histological scores showed less fibrosis of the IFP in the HA injection group than in the control group at 30 km run. Strenuous running exercise may cause fibrosis in the IFP of runners. Intraarticular HA injection will inhibit arthrofibrosis of painful runners.
本研究的目的是确定剧烈跑步运动是否会在膝关节髌下脂肪垫（IFP）中产生大量纤维化，以及关节内注射透明质酸（HA）是否能在大鼠跑步模型中保护IFP中的纤维化。使用Wistar大鼠在倾斜度为5%的啮齿动物跑步机上进行剧烈跑步运动。运动后，HA注射组每周一次将透明质酸注入右膝关节。在左膝的治疗对照组中，不进行注射。此外，跑步运动组和久坐对照组不接受HA注射。在1周（5公里）、2周（10公里）、3周（15公里）和6周（30公里）后，对IFP进行组织学和免疫组织化学分析。在每个跑步阶段，IFP中的炎症反应不明显。在剧烈跑步过程中，炎症细胞浸润量略有但显著增加。在30公里阶段，胶原纤维量显著增加。组织学评分显示，在30公里跑步时，HA注射组IFP的纤维化程度低于对照组。剧烈跑步运动可能会导致跑步者的IFP纤维化。关节内注射HA将抑制疼痛跑步者的关节纤维化。
