Valuev I L, Vanchugova L V, Talyzenkov Iu A, Valuev L I, Valueva T A
Prikl Biokhim Mikrobiol. 2009 Sep-Oct;45(5):527-31.
Trypsin was immobilized on polymeric carriers with low critical solution temperature (LCST). Homopolymer of N,N-diethylacrylamide (DEAA), random copolymers of DEAA and acrylamide (AA), and block copolymers polyDEAA-polyAA were used as the carriers. It was shown that at a temperature above LCST all carriers have a conformation change and trypsin's polymeric derivatives precipitate. The maximal activity after phase transition keeps trypsin, immobilized on polyDEAA block in polyDEAA-polyAA block-copolymer.
将胰蛋白酶固定在具有低临界溶解温度（LCST）的聚合物载体上。使用N，N-二乙基丙烯酰胺（DEAA）的均聚物、DEAA与丙烯酰胺（AA）的无规共聚物以及聚DEAA-聚AA嵌段共聚物作为载体。结果表明，在高于LCST的温度下，所有载体都会发生构象变化，并且胰蛋白酶的聚合物衍生物会沉淀。相变后的最大活性保留了固定在聚DEAA-聚AA嵌段共聚物中聚DEAA嵌段上的胰蛋白酶。
