[美拉芬对莱茵衣藻细胞中叶绿体伴侣蛋白HSP70B表达及光合色素的影响]

[The impact of melafen on the expression of chloroplastic chaperone protein HSP70B and photosynthetic pigments in cells of Chlamydomonas reinhardtii].

作者信息

Ermokhina O V, Belkina G G, Oleskina Iu P, Fattakhov S G, Iurina N P

出版信息

Prikl Biokhim Mikrobiol. 2009 Sep-Oct;45(5):612-7.

PMID:19845296

Abstract

The effects of growth regulator of the new generation-melamine salt of bis(oxymethyl)phosphine acid (melafen)--on culture growth, pigment and protein content, and the induction of protective chloroplastic chaperone HSP70B in Chlamydomonas reinhardtii CW15 cells were studied. Melafen exhibited 10-30% growth inhibition at 10(-9)-10(-2)% concentration. At 10(-9)-10(-4)% of melafen electrophoretic concentration, the pattern of cellular proteins was similar to the control. The alterations in protein content of algae cells were detected only at 10(-2)% concentration. The content of chlorophyll and carotenoids in melafen-treated cells was 17-40% lower than in the control. Melafen at 10(-9)-109-2)% concentration inhibited HSP70B induction by 39-43% compared to untreated cells. The potential mechanism of melafen effect might involve its influence on nuclear gene expression.

摘要

研究了新一代生长调节剂——双（羟甲基）膦酸三聚氰胺盐（melafen）对莱茵衣藻CW15细胞培养生长、色素和蛋白质含量以及保护性叶绿体伴侣蛋白HSP70B诱导的影响。在10^(-9)-10^(-2)%浓度下，melafen表现出10%-30%的生长抑制。在10^(-9)-10^(-4)%的melafen电泳浓度下，细胞蛋白质模式与对照相似。仅在10^(-2)%浓度下检测到藻类细胞蛋白质含量的变化。经melafen处理的细胞中叶绿素和类胡萝卜素的含量比对照低17%-40%。与未处理细胞相比，在10^(-9)-10^(-2)%浓度下，melafen抑制HSP70B诱导39%-43%。melafen作用的潜在机制可能涉及其对核基因表达的影响。