两种烟草花叶病毒之间运动蛋白基因的转移：对局部病斑形成的影响。

Transfer of the movement protein gene between two tobamoviruses: influence on local lesion development.

作者信息

Nejidat A, Cellier F, Holt C A, Gafny R, Eggenberger A L, Beachy R N

机构信息

Department of Biology, Washington University, St. Louis, Missouri 63130.

出版信息

Virology. 1991 Jan;180(1):318-26. doi: 10.1016/0042-6822(91)90036-b.

DOI:10.1016/0042-6822(91)90036-b

PMID:1984654

Abstract

The effects of transfer of the movement gene between the tobamoviruses tobacco mosaic virus (TMV) and tobacco mild green mosaic virus (TMGMV) were studied. The movement protein (MP) gene of TMGMV was cloned into an infectious cDNA of TMV to build the recombinant virus V23. V23, like TMV and TMGMV, caused systemic infection in Nicotiana tabacum Xanthi. In N. sylvestris V23 and TMV spread systemically although TMGMV produces necrotic local lesions on this host. V23 and TMV cause systemic infection on tomato plants while TMGMV does not infect tomato. In Xanthi nc plants, V23 produced necrotic local lesions similar in size to those produced by TMGMV. On the other hand in transgenic Xanthi nc tobacco plants that express a gene encoding the MP of TMV the necrotic lesions produced by V23 and TMGMV were similar in size to those produced by TMV. These results indicate that the size of necrotic lesions produced by TMGMV and TMV on Xanthi nc plants is influenced by the MP gene.

摘要

研究了烟草花叶病毒（TMV）和烟草轻绿花叶病毒（TMGMV）之间运动基因转移的影响。将TMGMV的运动蛋白（MP）基因克隆到TMV的感染性cDNA中，构建重组病毒V23。V23与TMV和TMGMV一样，在烟草品种Xanthi中引起系统感染。在野生烟草中，V23和TMV能系统传播，尽管TMGMV在此宿主上产生坏死性局部病斑。V23和TMV能在番茄植株上引起系统感染，而TMGMV不能感染番茄。在Xanthi nc植株中，V23产生的坏死性局部病斑大小与TMGMV产生的相似。另一方面，在表达编码TMV MP基因的转基因Xanthi nc烟草植株中，V23和TMGMV产生的坏死病斑大小与TMV产生的相似。这些结果表明，TMGMV和TMV在Xanthi nc植株上产生的坏死病斑大小受MP基因影响。