Laboratory for Advanced Separations Technologies, Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO 80401, USA.
Anal Chim Acta. 2009 Nov 3;654(1):59-63. doi: 10.1016/j.aca.2009.04.032. Epub 2009 May 4.
A two-step HPLC separation that combines isocratic and gradient elution has been developed for the analysis of trace impurities in fatty alcohol ethoxylates (FAEs). Quantitative estimates of the early eluting impurities were obtained from a calibration curve based on the responses of an evaporative light scattering detector (ELSD). Isocratic conditions were used to elute the impurities and gradient conditions for the main FAE components. In this manner, an acceptable compromise was obtained between resolution and analysis time. Fractions were collected and the impurities identified using MALDI-TOF mass spectrometry.
已开发出两步 HPLC 分离法，将等度洗脱和梯度洗脱相结合，用于分析脂肪醇乙氧基化物 (FAE) 中的痕量杂质。通过基于蒸发光散射检测器 (ELSD) 响应的校准曲线，可从定量估算出早期洗脱杂质的含量。等度条件用于洗脱杂质，梯度条件用于主要 FAE 成分。通过这种方式，可以在分辨率和分析时间之间取得可接受的折衷。使用 MALDI-TOF 质谱法收集馏分并鉴定杂质。
