IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Anal Chim Acta. 2009 Nov 3;654(1):71-6. doi: 10.1016/j.aca.2009.06.043. Epub 2009 Jun 23.
Adsorption equilibrium of fructose, glucose and sucrose was evaluated on sulfonated poly(styrene-co-divinylbenzene) cation-exchange resins. Two types of resins were used: potassium (K+) gel-type and sodium (Na+) macroporous resins. Influence of the cation and effect of the resin structure on adsorption were studied. The adsorption isotherms were determined by the static method in batch mode for mono-component and multi-component sugar mixtures, at 25 and 40 degrees C, in a range of concentrations between 5 and 250 g L(-1). All adsorption isotherms were fitted by a linear model in this range of concentrations. Sugars were adsorbed in both resins by the following order: fructose > glucose > sucrose. Sucrose was more adsorbed in the Na+ macroporous resin, glucose was identically adsorbed, and fructose was more adsorbed in the K+ gel-type resin. Data obtained from the adsorption of multi-component mixtures as compared to the mono-component ones showed a competitive effect on the adsorption at 25 degrees C, and a synergetic effect at 40 degrees C. The temperature increase conducted to a decrease on the adsorption capacity for mono-component sugar mixtures, and to an increase for the multi-component mixtures. Based on the selectivity results, K+ gel-type resin seems to be the best choice for the separation of fructose, glucose and sucrose, at 25 degrees C.
采用磺化的苯乙烯-二乙烯基苯共聚阳离子交换树脂研究了果糖、葡萄糖和蔗糖在树脂上的吸附平衡。使用了两种类型的树脂：钾（K+）凝胶型和钠（Na+）大孔树脂。研究了阳离子的影响以及树脂结构对吸附的影响。在 25 和 40°C 的温度范围内，在 5 到 250 g/L 的浓度范围内，通过静态分批法测定了单一组分和多组分糖混合物的吸附等温线。在该浓度范围内，所有吸附等温线均通过线性模型拟合。在两种树脂中，糖的吸附顺序为：果糖>葡萄糖>蔗糖。在 Na+大孔树脂中，蔗糖的吸附量更大，葡萄糖的吸附量相同，而在 K+凝胶型树脂中，果糖的吸附量更大。与单一组分相比，从多组分混合物的吸附数据中可以看出，在 25°C 下存在竞争吸附效应，而在 40°C 下存在协同吸附效应。温度升高会导致单一组分糖混合物的吸附容量降低，而多组分混合物的吸附容量增加。基于选择性结果，在 25°C 下，K+凝胶型树脂似乎是分离果糖、葡萄糖和蔗糖的最佳选择。
