Department of Allergy, University Hospital La Paz, Madrid, Spain.
Int Arch Allergy Immunol. 2010;151(4):331-5. doi: 10.1159/000250441. Epub 2009 Oct 22.
Since raw egg may cause digestive toxic infections, we assessed whether pasteurized raw egg white is as reliable as fresh raw egg white in the diagnosis of egg allergy.
Thirty-two egg-allergic children were challenged with both pasteurized and fresh raw egg white. Open challenges were carried out with increasing doses of pasteurized raw egg white and fresh raw egg white administered every 60 min.
Eleven children (34.4%) had positive challenges with pasteurized raw egg white. Twenty-one children (65.62%) who tolerated pasteurized raw egg white also had a negative challenge with fresh raw egg white. If the challenge with pasteurized raw egg white resulted positive, the study was stopped. The protein profile and IgE-binding capacity of both pasteurized and fresh egg white were almost identical as observed by SDS-PAGE and IgE immunoblotting. In the IgE immunoblotting-inhibition and ImmunoCAP-inhibition assays, both extracts behaved in a similar way.
We did not find any relevant allergenic differences between fresh and pasteurized egg white. This study supports the use of pasteurized egg white in the diagnosis of allergy to fresh raw egg proteins.
由于生鸡蛋可能会引起消化道中毒感染，我们评估了巴氏杀菌生蛋清是否与新鲜生蛋清一样可靠，可用于诊断鸡蛋过敏。
32 名鸡蛋过敏的儿童接受了巴氏杀菌生蛋清和新鲜生蛋清的挑战。开放性挑战采用逐渐增加剂量的巴氏杀菌生蛋清和新鲜生蛋清进行，每 60 分钟给药一次。
11 名儿童（34.4%）对巴氏杀菌生蛋清的挑战呈阳性。21 名（65.62%）耐受巴氏杀菌生蛋清的儿童对新鲜生蛋清的挑战也呈阴性。如果巴氏杀菌生蛋清的挑战呈阳性，则停止研究。SDS-PAGE 和 IgE 免疫印迹观察到巴氏杀菌和新鲜蛋清的蛋白图谱和 IgE 结合能力几乎相同。在 IgE 免疫印迹抑制和 ImmunoCAP 抑制检测中，两种提取物的行为方式相似。
我们没有发现新鲜和巴氏杀菌蛋清之间存在任何相关的变应原差异。本研究支持在诊断对新鲜生蛋清蛋白过敏时使用巴氏杀菌蛋清。
