Samuelson J, Lerner E, Tesh R, Titus R
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
J Exp Med. 1991 Jan 1;173(1):49-54. doi: 10.1084/jem.173.1.49.
To development a reliable murine model of Leishmania braziliensis braziliensis infection, parasites were injected into BALB/c mice in the presence of phlebotomine sand fly salivary gland lysates, which have previously been shown to greatly increase the infectivity of L. major in mice. When injected with salivary gland lysates, L. braziliensis braziliensis produced progressively enlarging cutaneous nodules, containing many macrophages filled with Leishmania amastigotes. In contrast, L. braziliensis injected without gland extracts produced small and rapidly regressing lesions. Isoenzyme analysis, monoclonal antibodies, and the polymerase chain reaction with L. braziliensis-specific oligonucleotide primers and probes confirmed that parasites causing the lesions were L. braziliensis.
为了建立一种可靠的巴西利什曼原虫感染小鼠模型，在存在白蛉唾液腺裂解物的情况下将寄生虫注射到BALB/c小鼠体内，此前已证明该裂解物可大大提高硕大利什曼原虫在小鼠中的感染性。当注射唾液腺裂解物时，巴西利什曼原虫产生逐渐增大的皮肤结节，其中含有许多充满利什曼原虫无鞭毛体的巨噬细胞。相比之下，未注射腺体提取物的巴西利什曼原虫产生小的且迅速消退的病变。同工酶分析、单克隆抗体以及使用巴西利什曼原虫特异性寡核苷酸引物和探针的聚合酶链反应证实，引起病变的寄生虫是巴西利什曼原虫。
