Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Mol Cell Biochem. 2010 Apr;337(1-2):77-81. doi: 10.1007/s11010-009-0287-y. Epub 2009 Oct 23.
Histamine is a potent mediator in allergic inflammatory processes and is released by basophils and mast cells. The aim of this study was to investigate the effect of histamine on in vitro migration of human fetal lung fibroblasts (HFL-1) to human plasma fibronectin (HFn), a chemoattractant. Using the blindwell chamber technique, histamine alone had no chemotactic activity. However, histamine augmented HFn-induced HFL-1 migration at concentrations ranging between 0 and 10(-7) M (290.6 +/- 20.8%) (P < 0.05). The concentration-response was bell-shaped. The effect of histamine increased with time. The stimulatory effect of histamine on HFL-1 migration was inhibited by an H4 receptor antagonist, JNJ7777120 (10(-5) M). Histamine's effect was also inhibited by pertussis toxin (50 ng/ml), showing that the effect was mediated by the H4 receptor. This study demonstrated that histamine has the potential to stimulate human lung fibroblast migration, and thus may contribute to regulation of wound healing and the development of fibrotic disorders of the lung.
组织胺是过敏炎症过程中的一种有效介质，由嗜碱性粒细胞和肥大细胞释放。本研究的目的是研究组织胺对人胎肺成纤维细胞（HFL-1）向人血浆纤维连接蛋白（HFn）的体外迁移的影响，HFn 是一种趋化因子。采用盲室技术，组织胺本身没有趋化活性。然而，组织胺在 0 至 10(-7) M 浓度范围内增强了 HFn 诱导的 HFL-1 迁移（290.6 +/- 20.8%）（P < 0.05）。浓度反应呈钟形。组织胺的作用随时间而增加。H4 受体拮抗剂 JNJ7777120（10(-5) M）抑制了组织胺对 HFL-1 迁移的刺激作用。百日咳毒素（50 ng/ml）也抑制了组织胺的作用，表明该作用是由 H4 受体介导的。本研究表明，组织胺有可能刺激人肺成纤维细胞迁移，从而可能有助于调节伤口愈合和肺部纤维化疾病的发展。
