[弓形虫速殖子在人包皮成纤维细胞和人宫颈癌上皮细胞中的体外培养]
[In vitro culture of Toxoplasma gondii tachyzoites in HFF and HeLa cells].
作者信息
Wu Liang, Zhang Qiu-Xia, Li Ting-Ting, Chen Sheng-Xi, Cao Jian-Ping
机构信息
School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Jun;27(3):229-31.
OBJECTIVE
To study the proliferation of Toxoplasma gondii RH strain tachyzoites in human foreskin fibroblast (HFF) cells and HeLa cells.
METHODS
HFF cells and HeLa cells were cultured in 35 mm cell culture dishes with glass cover slip. Confluent cells were co-cultured with tachyzoites which purified by 3 microm filter membrane. At 0.5, 1, 2, 4, 8, 12, 24, 36, 48, 72, and 96 h after co-culture, the invasion of tachyzoites into the cells and proliferation in cells were observed with Giemsa staining.
RESULTS
In 4 h after co-culture, there were dozens of T. gondii tachyzoites in the HFF cells. At 24 h many pseudocysts emerged. At 72 h most of the cells were destroyed by tachyzoites. While cultured in HeLa cells for 8 h, there were only 3-5 tachyzoites in one cell, and pseudocysts emerged at 48 h. At 96 h after co-culture, most cells were destroyed.
CONCLUSION
Toxoplasma gondii tachyzoites can be cultured in HFF cells and HeLa cells. The proliferation of tachyzoites in HFF cells was quicker than that in HeLa cells.
目的
研究弓形虫RH株速殖子在人包皮成纤维细胞（HFF）和HeLa细胞中的增殖情况。
方法
将HFF细胞和HeLa细胞接种于含盖玻片的35 mm细胞培养皿中培养。待细胞汇合后，与经3μm滤膜纯化的速殖子共培养。共培养0.5、1、2、4、8、12、24、36、48、72和96 h后，吉姆萨染色观察速殖子侵入细胞及在细胞内的增殖情况。
结果
共培养4 h后，HFF细胞内可见数十个弓形虫速殖子。24 h时出现许多假包囊。72 h时大部分细胞被速殖子破坏。在HeLa细胞中培养8 h时，一个细胞内仅有3 - 5个速殖子，48 h时出现假包囊。共培养96 h后，大部分细胞被破坏。
结论
弓形虫速殖子可在HFF细胞和HeLa细胞中培养。速殖子在HFF细胞中的增殖速度比在HeLa细胞中快。
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