用两种有机试剂增强 GC 丰富 DNA 的扩增。
Enhanced amplification of GC-rich DNA with two organic reagents.
机构信息
HIT Bio-X Centre for Systems Biotechnology, Harbin Institute of Technology, Weihai, China.
出版信息
Biotechniques. 2009 Sep;47(3):775-9. doi: 10.2144/000113203.
PMID:19852763
Abstract
Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.
摘要
GC 丰富 DNA 序列的扩增在全球范围内仍然是一项艰巨的任务。研究发现，在扩增 104 个随机选择的 GC 含量为 60-80%、长度为 700-800bp 的富含 GC 的人类 DNA 序列时，两种常用且廉价的试剂——乙二醇和 1,2-丙二醇——比甜菜碱更有效。
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