College of Agriculture, University of Kentucky, Lexington, KY 40506, USA.
Toxicon. 2010 Feb-Mar;55(2-3):612-8. doi: 10.1016/j.toxicon.2009.10.018. Epub 2009 Oct 21.
Venom production facilities keep established colonies of captive snakes to obtain venom for research and antiserum production. Due to strict regulations of importation, some of these colonies are formed with only a small number of initial animals and consist of closely related individuals (sometimes siblings). To understand the effect of long-term captivity on the venom composition and its impact on antiserum production, we analyzed 15 long-term captive Naja naja (Pakistan) originating from two separate venom production colonies using liquid chromatography-mass spectrometry and electrophoresis. The chromatogram produced from each individual cobra venom was found to be different. When the protein molecular masses of the peaks were identified, it was found that all the venoms consisted of the same protein composition, but the concentration of the proteins were different. Although three-finger toxins and phospholipase A(2) enzymes are the major toxic components present in these venoms, there was a clear difference in the amounts of each individual isoform. Such variation may affect the ability of antivenoms in neutralizing the toxic components of the wild type venom.
毒液生产设施利用圈养的蛇类来获取毒液，以进行研究和抗血清生产。由于进口的严格规定，这些毒液生产设施中的一些仅由少量初始动物组成，且这些动物亲缘关系密切（有时是兄弟姐妹）。为了了解长期圈养对毒液成分的影响及其对抗血清生产的影响，我们使用液相色谱-质谱联用和电泳技术分析了来自两个不同毒液生产设施的 15 条长期圈养的 Naja naja（巴基斯坦）。发现每条眼镜蛇毒液产生的色谱图均不同。当鉴定峰的蛋白质分子量时，发现所有毒液均由相同的蛋白质组成，但蛋白质的浓度不同。尽管三指毒素和磷脂酶 A(2)酶是这些毒液中的主要毒性成分，但每种同工酶的含量存在明显差异。这种差异可能会影响抗毒液中和野生型毒液中有毒成分的能力。
