流感嗜血杆菌脂寡糖突变对中耳和内耳的影响。
Effect of lipooligosaccharide mutations of Haemophilus influenzae on the middle and inner ears.
作者信息
Schachern Patricia A, Tsuprun Vladimir, Wang Beinan, Apicella Michael A, Cureoglu Sebahattin, Paparella Michael M, Juhn Steven K
机构信息
Department of Otolaryngology, University of Minnesota, Minneapolis, MN, United States.
出版信息
Int J Pediatr Otorhinolaryngol. 2009 Dec;73(12):1757-60. doi: 10.1016/j.ijporl.2009.09.034. Epub 2009 Oct 22.
OBJECTIVE
The purpose of this study was to determine the virulence of nontypeable Haemophilus influenzae 2019 (NTHi 2019) and its two lipooligosaccharide (LOS) mutant strains, B29 (gene htrB) and DK1 (gene rfaD), and compare their effect on the middle ear, round window membrane, and inner ear.
RESULTS
Fifteen chinchillas were divided into three equal groups and their bullas inoculated bilaterally with 0.5 ml of 10(2)CFU/ml of parent NTHi 2019, B29 or DK1 mutant strains. Two days after inoculation all animals had otitis media and inflamed middle ear mucosa. There was a trend of greater thickness and infiltration of the round window membrane in animals inoculated with the wild-type NTHi strain compared to the mutant strains and a significant increase in both inflammatory cell infiltration and bacteria presence in the scala tympani area of the inner ear. Strial edema was only observed in the wild-type-inoculated group.
CONCLUSIONS
LOS mutants of NTHi appear to have a reduced ability to pass through the round window membrane resulting in less inner ear inflammation and pathological changes.
目的
本研究旨在确定2019年不可分型流感嗜血杆菌（NTHi 2019）及其两种脂寡糖（LOS）突变株B29（基因htrB）和DK1（基因rfaD）的毒力，并比较它们对中耳、圆窗膜和内耳的影响。
结果
将15只龙猫分成三组，每组数量相等，通过双侧鼓泡接种0.5毫升浓度为10(2)CFU/毫升的亲本NTHi 2019、B29或DK1突变株。接种两天后，所有动物均患中耳炎且中耳黏膜发炎。与突变株相比，接种野生型NTHi菌株的动物的圆窗膜有更厚和浸润更明显的趋势，且内耳鼓阶区域的炎性细胞浸润和细菌存在均显著增加。仅在接种野生型的组中观察到血管纹水肿。
结论
NTHi的LOS突变株穿过圆窗膜的能力似乎降低，导致内耳炎症和病理变化减少。
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