Department of Dermatology, Medical School of Sao Paulo University, Sao Paulo, SP, Brazil.
J Med Virol. 2009 Dec;81(12):2007-11. doi: 10.1002/jmv.21624.
Human papillomavirus is a DNA virus that includes 118 genotypes. HPV16 is responsible for 80% of cervical cancer in women. Men are important reservoirs and major transmitters of HPV to their partners. The aim of this study was to detect HPV DNA and to determine the prevalence of HPV types 6, 11, 16, and 18 in urine samples of men infected with HIV-1. This study included 223 patients infected with HIV-1 from the Center of Reference on HIV/AIDS (CRT-SP) and an outpatient clinic of HIV. Urine samples were collected and after DNA extraction real-time PCR was performed for detection of HPV DNA. Positive samples were then tested by conventional PCR using type-specific primers for the four HPV types. A total of 223 men infected with HIV-1 were tested, 81% of whom were on HAART. Four (5.8%) were positive for HPV6, 18 (26.1%) were positive for HPV11, 22 (31.9%) were positive for HPV16 and five (7.2%) were positive for HPV18 by conventional PCR. Twenty (29%) patients had other HPV types and five patients (1.5%) had multiple types. The mean T CD4+cells count was 517 and 441 cells/mm(3) (P = 0.30), in HPV negative and positive men, respectively. The HIV viral load was higher in the HPV negative group than for in the men with HPV (P = 0.0002). A 30.9% prevalence of HPV was found in asymptomatic urine samples of men infected with HIV-1. This study suggests that urine may be a useful specimen for HPV screening.
人乳头瘤病毒是一种 DNA 病毒，包含 118 种基因型。HPV16 导致 80%的女性宫颈癌。男性是 HPV 的重要储存库和主要传播者，将 HPV 传播给其伴侣。本研究旨在检测感染 HIV-1 的男性尿液样本中的 HPV DNA，并确定 HPV 6、11、16 和 18 型的流行率。本研究包括来自艾滋病毒参考中心（CRT-SP）和艾滋病毒门诊诊所的 223 名感染 HIV-1 的患者。采集尿液样本，提取 DNA 后进行实时 PCR 检测 HPV DNA。阳性样本用针对四种 HPV 型的型特异性引物进行常规 PCR 检测。共检测了 223 名感染 HIV-1 的男性，其中 81%接受了高效抗逆转录病毒治疗。4 名（5.8%）HPV6 阳性，18 名（26.1%）HPV11 阳性，22 名（31.9%）HPV16 阳性，5 名（7.2%）HPV18 阳性。20 名（29%）患者有其他 HPV 型，5 名（1.5%）患者有多种类型。HPV 阴性和阳性男性的平均 CD4+T 细胞计数分别为 517 和 441 个细胞/mm3（P = 0.30）。HPV 阴性组的 HIV 病毒载量高于 HPV 阳性组（P = 0.0002）。在感染 HIV-1 的无症状男性尿液样本中发现 HPV 的流行率为 30.9%。本研究表明，尿液可能是 HPV 筛查的有用标本。
