Roomi M Waheed, Roomi Nusrath W, Kalinovsky Tatiana, Rath Matthias, Niedzwiecki Aleksandra
Dr Rath Research Institute, Oncology Division, Santa Clara, CA 95050, USA.
Tumori. 2009 Jul-Aug;95(4):508-13. doi: 10.1177/030089160909500417.
Lung cancer, a leading cause of cancer death, is associated with exposure to inhalation carcinogens, most commonly those found in tobacco smoke. We investigated the in vivo effect of dietary supplementation with a nutrient mixture containing lysine, proline, arginine, ascorbic acid, green tea extract, N-acetyl cysteine, selenium, copper and manganese on the development of urethane-induced lung tumors in male A/J mice.
After one week of isolation, seven-week-old male A/J mice (n = 25) weighing 17-19 g were randomly divided into three groups: group A (n = 5), group B (n = 10), and group C (n = 10). Mice in groups B and C were each given a single intraperitoneal injection of urethane (1 mg/g body weight) in saline, whereas group A mice received an injection of saline alone. Groups A and B were fed a regular diet, whereas group C was fed the same diet supplemented with 0.5% nutrient mixture. After 20 weeks, mice were sacrificed, lungs were excised and weighed, and tumors were counted and processed for histology.
Urethane-challenged mice developed tumors. However, the mean number of tumors and the mean lung weights in the mice on the supplemented diet were significantly reduced, by 49% (P < 0.0001) and 18% (P = 0.0025), respectively, compared to mice on the control diet. We observed neither significant differences in body weight gains nor in diet consumption among the mice. Pulmonary lesions were morphologically similar for both the groups (adenomas), but lesions were smaller in the test group.
The results suggest that nutrient mixture has inhibitory potential on the development of mouse lung tumors induced by urethane.
肺癌是癌症死亡的主要原因，与吸入致癌物有关，最常见的是烟草烟雾中的致癌物。我们研究了在雄性A/J小鼠中，用含有赖氨酸、脯氨酸、精氨酸、抗坏血酸、绿茶提取物、N-乙酰半胱氨酸、硒、铜和锰的营养混合物进行膳食补充对氨基甲酸乙酯诱导的肺肿瘤发生的体内影响。
隔离一周后，将体重17 - 19克的7周龄雄性A/J小鼠（n = 25）随机分为三组：A组（n = 5）、B组（n = 10）和C组（n = 10）。B组和C组小鼠分别腹腔注射一次氨基甲酸乙酯（1毫克/克体重）的生理盐水溶液，而A组小鼠仅注射生理盐水。A组和B组喂食常规饮食，而C组喂食添加了0.5%营养混合物的相同饮食。20周后，处死小鼠，切除肺并称重，计数肿瘤并进行组织学处理。
接受氨基甲酸乙酯攻击的小鼠发生了肿瘤。然而，与对照饮食组小鼠相比，补充饮食组小鼠的平均肿瘤数量和平均肺重量分别显著降低了49%（P < 0.0001）和18%（P = 0.0025）。我们观察到小鼠之间的体重增加和饮食消耗均无显著差异。两组的肺部病变在形态上相似（腺瘤），但试验组的病变较小。
结果表明，营养混合物对氨基甲酸乙酯诱导的小鼠肺肿瘤发生具有抑制潜力。
